The Orbit Discovery Affinity Screening platform offers a proprietary technology enabling the construction of massive bead-based libraries with peptides displayed on their surface. Each bead carries a unique DNA sequence, which is translated into peptide using in vitro transcription translation (IVTT) performed in an emulsion (emIVTT). Each droplet of the emulsion acts as a micro-compartment which allows translation of the DNA on each individual bead to occur separately. The result is a library of unique beads each displaying thousands of copies of peptide, providing massive chemical diversity and the ability to perform rapid screens for high affinity binders against soluble or more complex target proteins, such as GPCRs and ion channels.
The platform is flexible, allows for different library structures (linear, cyclic, helical), peptide lengths, diversity, and chemistries. The high diversity that can be covered produces an abundance of hits that can be further optimised as therapeutic candidates. Binders in the nanomolar range can be detected direct from the screen, weak binders in the micromolar range can also be identified. Binders are analysed for conserved motifs and structures, these can be synthesised, and binding confirmed (peptide microarray, thermal shift, SPR or cell assays can be performed) prior to taking into further medicinal chemistry. Alternatively, binders can be used to design smaller more refined libraries that can be taken into cell based functional screening
Key benefits of Orbit's affinity screening
Sensitivity ~ nM to uM binders
Highly diversity massive libraries